EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. . When the user applies a set temperature of 37°C, the top is heated to 37. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. Bookings are free, but you will need to use iLab to book. . . Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 4. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. ISM Equipment Classification (Group 1, Class A) This equipment is. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Samples were measured together with a corresponding reference solvent contained in a. SpectraMax fluorescence microplate reader with SoftMax® Pro Software, is a quick, sensitive detection method for proteins. Spectramax M5 plate reader (96/384 format) Multimode photometrics for endpoint or kinetic measurements. These robust readers have been placed in labs from. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. The cells were then washed and staining quantitation was performed using a fluorescent plate reader (SpectraMax M5 Molecular Devices, Sunnyvale, CA) at 492/520 excitation emission wavelengths. When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). 95. Note: In this user guide, SpectraMax ® refers to several SpectraMax . . Pick the best speed, sensitivity, and price for your needs. . The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Published Dec 16, 2020 | Updated Dec 16, 2020. spr SpectraMax iD5 SpectraTest ABS2 v1. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. STAKMAX. 304 signal level saturation. . Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 1) SpectraMax Glo Steady Luc Reporter Assay (6. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. We would like to show you a description here but the site won’t allow us. . Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. 72 MB. 4. . Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. Add to Cart. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M5 Multi-Mode Microplate Reader. 7. This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. D 1 1. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Turn on the microplate reader. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. . Wavelength optimization. spr SpectraMax iD3 SpectraTest ABS2 v1. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. I have been using the SpectraMax M5 since 2011. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. . The SpectraMax M5 is a microplate reader that can be used for various applications,. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. 306 plate air cal. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. For example, microplates containing different samples can be read using the312 gain calibration failed. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Access this by going to Help >. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. Description 1. While they play an important role in generating data from a wide range of. clicked with the SpectraMax L Microplate Reader. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 5. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 0 out of 5 $ 17,999 $ 16,995. Molecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. Supplier: Molecular Devices. Italic and boldface type are used for emphasis. Step 2. Customer Satisfaction is our number one goal All lab equipment in stock. 316 grating motor driver faulty. Fluorescent Polarization: Solid black plates, as above. The . Select the fluorescence option and read the total fluorescence. TR-FRET assay principle. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. . Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Purchase Overlord with the required number of. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Table of Contents. No accessories are needed and SoftMax® Pro. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. ; The top 3 importers of Spectramax are India with 365. 7°C and the bottom to 36. spr SpectraMax Plus 384. The drawing shows a standard microplate of 15 mm height covered with a lid. 1. Choose cables that meet the requirement described in this appendix. 305 reference level saturation. ScanLater Western Blot Detection Cartridge. Cell viability was assessed by CellTiterGlo (Promega) reagent 96 hours after beginning treatment, and luminescence was measured on a Spectramax M5 spectrophotometer. Examples: “Press carefully to engage,” “ Do not press down. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Some luminescence applications, such as gene reporter assays, may require a luminometer with photon counting detection for greater sensitivity, such as the SpectraMax M5, Analyst GT or Analyst HT, or LMax II. The assay window spanned four orders of magnitude. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. For more information, please visit us at Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The test solution was by diluting FCV to PBS/ Glycerol solutions (5 μM, 1. For example, microplates containing different samples can be read using theThe interval between reads was set at 15 s or 60 s, respectively. 72 MB. HEALTH AND SAFETY CONSIDERAIONS The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. Open the Softmax Pro software and select the FP option. Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Introduction. Table of Contents. D 1 1. Mass spectra were recorded using an HPLC-System Series 1100 coupled with ESI-single. . SpectraMax® M5/M5e/FlexStation® 3 Instrument Settings Instrument Wavelength Settings EXC Wavelength 575 nm EMS Wavelength 620 nm Auto Cutoff 610 nm Optimized SpectraMax® M5/M5e Settings Readings 10-50 PMT Auto Auto Calibrate On Transcreener® HTS is a universal, high throughput biochemical assayUser guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Optimal instrument settings are the same for all three readers. Choose from a three- (M3), four- (M4), orSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Page: 1/6. S. Spectral Range. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. 190-1000 nm, tunable in 1 nm increments. 1. I have been using the SpectraMax M5 since 2011. The IP-One assay can be used to characterize compounds that act on Gq-coupled receptors in either adherent or suspension cells. 多功能酶标仪 FlexStation3 快速动力学操. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 0200-7027. Exhibit with us {{>currencyLabel}} Back. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. It is available to all researchers. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. free tracer decreases, resulting in an. . . The Spectramax performs spectral scanning in 1 nm increments, and up to six wavelengths. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. Description 1. D 1. In either case. Five preferred detection modes. The day after, a final concentration of 1 μmol/L ARS1620, 20 nmol/L SMARTpool siRNA or the combination of the 2 was added in 5 replicates (wells). Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision Here, we present FluoroBrite™ DMEM, a cell culture media designed specifically for live-cell imaging that, when excited at wavelengths commonly used for FITC/GFP (i. Description 1. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . 303 unable to cal dark current. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 26 5039228D Note. SoftMax Pro. 00 USD. 7 nM EGF, the EC 80 concentration determined from. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. com A. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Molecular Devices SpectraMax M5 Multimode Microplate Reader. 305 reference level saturation. SpectraMax L Microplate Reader provides medium to high throughput. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. . Detection cartridge modularity allows for easy addition of new read modes within minutes without. 0 (IBM Corporation, Armonk, NY, USA). Cables. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. 01) + 0. Fix: While proper use of the Shake button is to hold the button down for the duration of the desired mixing time, repeated sequential clicking is now allowed. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 6 lbs. 检测模式包括： • 紫外-可见光 光吸收 (Abs) • 荧光强度 (FI) • 化学发光 (Lum) • 时间分辨荧光 (TRF) • 荧光偏振 (FP) SpectraMax M5e 在 M5 基础上通过了 Cisbio HTRF 检测认证而增加了应用范围。. 7°C and the bottom to 36. After 4 h, the fluorescence intensity of serum FITC-dextran was obtained at 480 nm excitation wavelength and 520 nm emission wavelength using a Spectramax M5 microplate reader (Molecular Devices). The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Fluorescence Microplate Reader Comparison Chart. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The absorbance and photo-luminescence was determined on a SpectraMax M5 plate reader via quartz cuvette using 1 × 10 10 particles per ml or lower and extrapolated when an optical density of 1 was. Microplate Reader Shelf. Step 4: Incubate at room temperature for 1 hour, protected from light. D 1 1. Step 1. . Read fluorescence polarization at room temperature (RT, 19–25°C). Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Lift up the lid over the chamber to insert or remove a cuvette. Add to Cart. Table of Contents. The plate reader. D 47 7. 5. Example: [Drawer]. Find regional support. The SpectraMax M5 is a microplate reader that can be used for various applications, such as ELISA, Western blot, or cell culture. Customer. In this. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Figure 2. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. In the M2, M5 and Plus 384, the light. Choose from a three- (M3), four- (M4), or Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Description. Weight. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. For example, microplates containing different samp les can be read using the same or Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 313 reference gain check fail. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The following document is intended to demonstrate setup of this instrument and provide representativeUsed together with the SpectraMax iD3 reader, the EarlyTox Cell Viability Assay Kits enable direct measurement of live and dead or apoptotic cells with a simple workflow and the increased throughput offered by a microplate format. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. SOFTmax PRO UserÕs Manual iii Contents Figures . Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. 6: bioNova científica, s. SpectraMax Microplate Readers. 6. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. $1,250. View the Molecular Devices websiteSpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. D 1. When the correct COM port is selected, the. DNA provides the same sensitivity as a spectrophotometer. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. SpectraMax Injector Cartridge with SmartInject™ Technology. 3: The cuvette chamber. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidePlace the plate into a SpectraMax M5 e plate reader (Molecular Devices). 3. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. The substrate solution (100 μL/well) was subsequently employed for 5-min reaction and the absorbance at 370 nm was monitored on a SpectraMax M5 plate reader (Molecular Devices). Highly selective for dsDNA over ssDNA or RNA. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Softmax Pro GxP Software 6. Proceed with the following steps to optimize the instrument: 1. 40266 Email: drugdiscoverytech@lifetech. Monday to Friday 7am - 5pm Pacific Time. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. e. 0 out of 5 $ 5,999. you can use a resource. TR-FRET assay. Maximizes fluorescent signal from a wide range of cell types by reducing cell wash artifacts. Sensitive fluorescent quantitation of DNA with the Quant-iT. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. Step 2. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. , ~488 nm), has the optical. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Offer Ends In: 18 Days 19: 38: 21. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The following document is intended to demonstrate setup of this instrument. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Read fluorescence polarization at room temperature (RT, 19–25°C). • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. The following document is. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. ) Click the "OK" button on SoftMax Pro Setup dialog box . Plate chamber design of the SpectraMax category 1 microplate readers. We would like to show you a description here but the site won’t allow us. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 5. SpectraMax ABS Plus SpectraTest ABS2 v1. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Features. . EarlyTox Live Cell Assay (6. 3: The cuvette chamber. 3. For example, microplates containing different samples can be read using theLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. 315 can't find zero order. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The dual monochromator optics allow the widest range of applications to be utilized for bior. Table of Contents. Refurbished. Select a rugged, lightweight model designed to be used in the field. 315 can't find zero order. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Cavicide (Warehouse, Cat # 79300360) 6. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. 0 mL). The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Choose from a three- (M3), four- (M4), or SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity,. . Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Table of Contents. Keywords: spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software,. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax ® Pro Software. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 1. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. ® reader to the computer. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). These lab workhorses are used in drug discovery, research, bioassay validation, and. Bottom-reading is usually recommended for cell-based assay with adherent cells. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. The following document is intended to demonstrate setup of this instrument. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). A preconfiguredAcme Revival: Long Live DevicesWe would like to show you a description here but the site won’t allow us. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. . The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 2. With optimized reagents, option to operate the readers. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. , ~488 nm), has the optical. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Here is a Molecular Devices Spectramax M5 Microplate Reader for sale. If you do not have an iLab account you must first make one: SpectraMax Paradigm Fluo rescence Polarization (FP) Detection Cartridge is a single slot detection cartridge and occupies one (1) slot. . New drivers take 6 days to write, existing drivers take 2 days to convert to Windows 7/10 compatibility. Cet appareil ISM est confomre à la norme NMB-001 du Canada. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). Formula accessors for reporting o The accessor!sectionname will return the name of the SoftMax Pro section containing the. Description 1. together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. . 0200-0729. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. Also refer to this Multimode Reader Comparison Chart. the IMAP Platform and smoothly transition into high-throughput screening. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. Five preferred modes for diverse bioresearch applications. The SoftMax® Touch Software is installed in. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Call 516-939-0888 to check current. 7°C (gradient heating design). SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The guide also covers the software features, the specifications, and the accessories of the systems. The drawing shows a standard microplate of 15 mm height covered with a lid. View regional contact information and Technical Support hours: Contact Us Hi Guest, welcome to. For example, microplates containing different samples can be read using the The interval between reads was set at 15 s or 60 s, respectively. We would like to show you a description here but the site won’t allow us. 0 out of 5 $ 15,999. Add to Cart. The. com A. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. Cavicide (Warehouse, Cat # 79300360) 6. 9000-0756. These robust readers have been placed in labs from Antarctica to the. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. 1. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Place the plate into a SpectraMax M5 e plate reader (Molecular Devices). Molecular Devices. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. 314 low lamp level warning. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. In the M2, M5 and Plus. 3: The cuvette chamber. , contact Molecular. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . Molecular Devices ®(MD) SpectraMax 340PC reader MD VersaMax™ Tunable Microplate readers MD SpectraMax® L Microplate Luminometers MD SpectraMax® M5 systems MD ®SpectraMax 96/384-well plate reader Gemini fluorescence plate reader Automation Hamilton Microlab® STAR™ liquid handling systemThe SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Refer to “Installing Detection Cartridges” in. e. Description 1. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel.